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How to Monitor and Minimize Off-Target Events During Genome Editing

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"How to Monitor and Minimize Off-Target Events During Genome Editing"

CRISPR-mediated genome editing is an extremely powerful tool that enables researchers to create different cellular models by removing, adding, or altering sections of a DNA sequence in the genome in a wide range of different cell types and gene loci.

While it can achieve high editing efficiencies, the CRISPR-Cas9 system can also cleave the target DNA at unintended locations (known as off-target events) that can result in undesired phenotypes or loss of functional gene activity, which is especially detrimental for therapeutic applications.

This white paper from Thermo Fisher Scientific discusses strategies to optimize the various factors that cause off-target events, describes an unbiased analysis system, TEG-seq, to help detect off-target events, and describes the use of this system to screen for a high-fidelity Cas9 mutant with improved specificity.

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