Research Library

The top resource for free research, white papers, reports, case studies, magazines, and eBooks.

Share Your Content with Us
on TradePub.com for readers like you. LEARN MORE
Achieve High Specificity and On-Target Efficiency with TrueCut HiFi Cas9 Protein

Request Your Free White Paper Now:

"Achieve High Specificity and On-Target Efficiency with TrueCut HiFi Cas9 Protein"

Off-target effects can have undesirable consequences in therapeutic CRISPR-Cas9 genome editing applications.

In this application note from Thermo Fisher Scientific, the authors detail their efforts to identify a Cas9 variant that would limit off-target edits, using a target-enriched GUIDE-seq (TEG-seq) to screen several Cas9 candidates. They present their development of a high-fidelity Cas9 protein, TrueCut HiFi Cas9, which was optimized for editing efficiency and showed higher fidelity than wild-type TrueCut Cas9 Protein v2 and other commercially available high-fidelity Cas9 proteins in a variety of cell types.


Offered Free by: Thermo Fisher Scientific
See All Resources from: Thermo Fisher Scientific

Recommended for Professionals Like You:

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.