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Optimizing Performance of Whole Transcriptome RNA-Seq Reference Materials

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"Optimizing Performance of Whole Transcriptome RNA-Seq Reference Materials"

Whole-transcriptome RNA-seq is one of the most effective methods for detecting genomic rearrangements in cancer. Enriching RNA samples with messenger RNA (mRNA) is an important step in sample preparation to avoid the muddling effects of less relevant RNAs. Poly-A selection targets the poly-A tails of mRNAs by binding them to oligo-dT sequences, but capture is not always optimal.

This whitepaper describes a study investigating whether extending the poly-A tails of clinically relevant fusion mRNAs in the Seraseq Fusion RNA Mix v4 reference material improves how efficiently they bind to oligo-dT columns during poly-A selection. The researchers found modest improvements in performance of reference materials after increasing the poly-A tail length.

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